Rabbit Anti-CDKN2A (Cyclin-Dependent Kinase Inhibitor 2A, p16INK4a) (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Cyclin-dependent kinase inhibitor 2A (CDKN2A), a 16kD protein belonging to the CDKN2 cyclin dependent kinase inhibitor family.
CDKN2A acts as a negative regulator of cell proliferation by interacting strongly with CDK4 and CDK6, thus inhibiting their ability to interact with cyclin D and to phosphorylate the retinoblastoma protein.
CDKN2A is an important tumor suppressor and defects in its production results in tumor formation in a wide range of tissues, including cutaneous malignant melanoma and Li-Fraumeni syndrome.
Applications
Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
19-amino acid peptide mapping near the N-terminus of human CDKN2A.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CDKN2A. Species Crossreactivity: mouse and rat
