Rat Anti-FLAG-Tag (DDDDK Epitope Tag, DYKDDDDK Epitope Tag, DYKDDDDK tag, ECS Epitope Tag, ECS tag, Enterokinase Cleavage Site Epitope Tag, Enterokinase Cleavage Site tag, FLAG) (FITC)
DYKDDDDK-Tag (ECS, Enterokinase Cleavage Site, FLAG, D Tags)
Applications
Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to DYKDDDDK Tag.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) when fused to either the amino or carboxyl terminal or at the internal site on target proteins.
