Rabbit Anti-I-TAC (ITAC, SCYB11, SCYB9B, Interferon-inducible T-cell alpha Chemoattractant, CXCL11) (Biotin)
I-TAC is a member of the CXC chemokine family, which signals through binding to CD183 (CXCR3), and is regulated by interferon gamma and beta. I-TAC is highly expressed by peripheral blood leucocytes, the pancreas and liver, and acts as a potent chemoattractant for IL-2 activated T cells, as well as a natural antagonist for CD195 (CCR5).
Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry, Functional Assays. Other applications not tested.
Recommended Dilution
ELISA: 0.5-2ug/ml Western Blot: 0.1-0.2ug/ml Immunohistochemistry: Paraffin. Antigen retrival using heat treatment prior to staining of paraffin sections is required. Functional Assays: 7-8ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human I-TAC.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human I-TAC.
