Mouse Anti-Lipoprotein Lipase (LDL) (HRP)
Lipoprotein lipase (LPL) is a member of the AB hydrolase superfamily, which plays a pivotal role in lipoprotein metabolism and transport, acting as the key enzyme in the hydrolysis of triglycerides and very low density lipoproteins (VLDLs), and the release of free fatty acids into peripheral tissues. A deficiency of LPL can result in hypertriglyceridemia, and many studies have focused on the critical role which LPL plays in the pathogenesis of atherosclerosis, and in particular the relationship between LPL and apolipoprotein E (ApoE), both of which are secreted in significant amounts by macrophages in developing arterial wall lesions. 214978 is a unique antibody which differentiates between monomeric inactive and dimeric active LPL, and binds to LPL sequences involved in LPL, LPL receptor, and heparin interactions.
214979 has been shown to inhibit the activity of human LPL (Chang et al. 1998), and is widely used in human studies.
Applications
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
ELISA: in a direct ELISA, or as the detection reagent in a sandwich ELISA with 214978 as the capture antibody. Western Blot: detects a band of ~53kD using partially purified LPL from postheparin plasma. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
Immunogen
Purified bovine milk lipoprotein lipase. Epitope: aa380-410
Form
Supplied as a liquid in PBS, 0.0095% MIT. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes bovine lipoprotein lipase. Species Crossreactivity: Chicken, guinea pig, human and rat. Does not react with mouse.
