215132-ML650
Clone Type
MonoclonalHost
MouseSource
HumanConjugate
MaxLight™650Isotype
IgG1Clone Number
K8Grade
Affinity PurifiedApplications
FC FLISA IF IP WBCrossreactivity
HuGene ID
27180Gene #
SIGLEC9Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeNotes
Preservative Free
BSA Free
Mouse Anti-Siglec 9 (Sialic Acid-binding Ig-like Lectin 9, Siglec 9, Protein FOAP-9, UNQ668, PRO1302) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
Siglec-9 (Sialic acid-binding Ig-like lectin 9), is a single pass type I membrane protein belonging to the immunoglobulin superfamily. Human Siglec-9 is broadly expressed in a range of human tissues with high expression on monocytes and low-level expression on neutrophils and subpopulations of NK, B, and T cells.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot and Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:25-1:200, 10ul labels 1x10e6 cells in 100ul Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to Siglec-9 fused to Fc region of human IgG.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human SIGLEC9.
