Mouse Anti-TCR, alpha, beta (T Cell Receptor, T-cell Receptor beta-1 Chain C Region, TRBC1, T-cell Receptor alpha Chain C Region, TRAC, TCRA) (MaxLight 650)
MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
TCRalpha/beta is expressed on peripheral blood T cells. Two distinct subpopulations of alpha/beta T cells appear in the chicken thymus subsequent to the appearance of gamma/delta T cells. These subpopulations were originally denoted as TCR2 and TCR3. These arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively.
Applications
Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunohistochemistry: frozen Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Specificity
Recognizes chicken TCR Alpha/Beta.
