Rat Anti-TIM-3 (Tim3, Timd3, T-cell Immunoglobulin Mucin 3, Havcr2) (FITC)
T-cell immunoglobulin mucin 3, a transmembrane glycoprotein and member of the immunoglobulin superfamily, is preferentially expressed by differentiated CD4+ Th1 cells and CD8+ Tc1 (cytotoxic) cells, but not by CD4+ Th2 cells or CD8+ Tc2 cells. Tim-3 acts as a negative regulator of Th1-mediated inflammatory diseases including GVHD, type I diabetes, and EAE (experimental autoimmune encephamyelitis), promotes immunological tolerance, and is a receptor for galectin-9, shown to induce cell death of Th1 cells in vitro. Tim-3 is also involved in the regulation of macrophage activation, acts as a phagocytic receptor, and has been shown to recognize apoptotic cells via the IgV domain. Studies have shown Tim-3 significantly inhibits the efficient phagocytosis of apoptotic cells by PEMs (peritoneal exudate Mac1+ cells) (Nakayama et al. 2009).
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: neat, 1:10, 10ul labels 1x10e6 cells in 100ul. Optimal dilutions to be determined by the researcher.
Hybridoma
Lymph node cells from immunized SD rats were fused with cells of the P3U1 myeloma cell line.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Tim-3-Ig, consisting of aa1-191 from mouse Tim-3, coupled with the Fc portion of mouse IgG2a.
Form
Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse TIM-3.
