Rabbit Anti-Presenilin 2 (PS-2, AD3LP, AD5, E5-1, STM-2, PSEN2, AD4, PS2, PSNL2, STM2)
Presenilin-2 is a multi-pass membrane protein and component of the gamma-secretase complex. 2 isoforms are produced by alternative splicing.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1ug/ml Immunohistochemistry (formalin fixed/paraffin embedded tissues): 1ug/ml. Heat mediated antigen retrieval is required. Immunohistochemistry (frozen): 1ug/ml. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa319-334 from human Presenilin-2. Species Sequence Homology: Mouse
Form
Supplied as a lyophilized powder in 5mg BSA, 0.9mg sodium chloride, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg sodium azide. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Presenilin-2. Species Crossreactivity: Rat. Predicted length of 448aa and MW of 50kD.
