Technical Data

216793
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2
Clone Number
14L697
Grade
Affinity Purified
Applications
FC IHC IP
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Notes
Preservative Free
Rat Anti-LPAM-1 (Integrin alpha4-beta7)

Integrin a4b7 is composed of a 150kD (a4 or CD49d) and a 130kD (b7) heterodimer, also known as CD49d/b7, or LPAM-1. It belongs to Ig superfamily found on majority of peripheral lymphocytes and subsets of thymocytes and bone marrow cells (including mast cell progenitors). Integrin a4b7 binds its ligands, VCAM-1 (CD106), MAdCAM-1 and fibronectin, and plays an important role in lymphocytes adhesion and helps direct the migration of blood lymphocytes to the intestine and associated lymphoid tissues.

Applications
Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, and Blocking. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Endotoxin: <0.002EU/ug
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
TK1 cells
Form
Supplied as a lyophilized powder in PBS. No preservative added. Reconstitute with sterile PBS.
Purity
Purified by Protein G affinity chromatography from culture supernatant.
Specificity
Recognizes mouse LPAM-1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
United States Biological | 4 Technology Way | Salem, MA 01970
Phone 800-520-3011 | Fax 978-594-8052 | Website www.usbio.net