Mouse Anti-PD-L2 (PDL-2, Programmed Cell Death 1 Ligand-2, Programmed Death Ligand 1, CD273, B7DC)
Programmed death ligand 2 (PD-L2), also named B7DC and butyrophilin-like protein, is a member of the B7 family of proteins that provide signals for regulating T cell activation and tolerance. Other family members include B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular V-type Ig-like and C-type Ig-like domains and short cytoplasmic domains. Among the family members, they share 20 - 40% amino acid sequence identity. The extracellular domains of mouse and human PD-L2 share approximately 72% amino acid sequence identity
Applications
Suitable for use in Flow Cytometry, Western Blot, and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:20-1:200 Western Blot: 1:500-1:1000 Immunohistochemistry (paraffin): 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human PD-L2
Form
Supplied as a lyophilized powder in PBS. Reconstitute with 200ul sterile PBS.
Purity
Purified by Protein G affinity chromatography from culture supernatant.
Specificity
Recognizes human PD-L2.
