Mouse Anti-CYP8B1 (Cytochrome P450, Family 8, Subfamily B, Polypeptide 1)
Implicated in hepatocellular carcinoma
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: Neat ELISA: Neat Immunohistochemistry: Neat. Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher.
Positive Control
ELISA: Peptide immunogen Western Blot: Human liver microsomes Immunohistochemistry: Formalin-fixed, paraffin-embedded Human liver
Hybridoma
Arg8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to aa486-494, QPSHDVRFR of human CYP8B1.
Form
Supplied as a liquid.
Specificity
Recognizes human CYP8B1. Species Crossreactivity: rat
