Rabbit Anti-ENO1 (2 phospho D glycerate hydro Lyase, 2-phospho-D-glycerate hydro-Lyase, Alpha Enolase, Alpha-Enolase, C myc promoter Binding Protein, C-myc promoter-Binding Protein, EC 4.2.1.11, eno1, ENO1L1, ENOA, Enolase 1 (alpha), Enolase 1 (alpha) like 1, Enolase 1, MBP 1, MBP-1, MBP1, MBPB1, MPB 1, MPB-1, MPB1, MYC promoter Binding Protein 1, NNE, Non neural enolase, Non-neural Enolase, Phosphopyruvate Hydratase, Plasminogen Binding Protein, Plasminogen-Binding Protein, PPH)
2 phospho D glycerate hydro Lyase, 2-phospho-D-glycerate hydro-Lyase, Alpha Enolase, Alpha-Enolase, C myc promoter Binding Protein, C-myc promoter-Binding Protein, EC 4.2.1.11, eno1, ENO1L1, ENOA, Enolase 1 (alpha), Enolase 1 (alpha) like 1, Enolase 1, MBP 1, MBP-1, MBP1, MBPB1, MPB 1, MPB-1, MPB1, MYC promoter Binding Protein 1, NNE, Non neural enolase, Non-neural Enolase, Phosphopyruvate Hydratase, Plasminogen Binding Protein, Plasminogen-Binding Protein, PPH,
Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production. MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
Applications
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry and Immunoprecipitation. Other applications not tested.
Recommended Dilutions
Western Blot: 1:500-1:3000 Immunohistochemistry (Paraffin): 1;50-1:200. Heat antigen nretrieval with 10mM citrate buffer pH 6.0 is recommended. Immunofluorescence (IC): 1:50-1:200 Immunoprecipitation: 1:50-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant protein corresponding to human ENO1.
Form
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide,|50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes endogenous levels of human ENO1. Species Crossreactivity: mouse and rat.
