Mouse Anti-CD34 (MaxLight 490)
MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.
Specific for canine homologue of CD34, a glycosylated type 1 transmembrane protein of ~110kD (McSweeney et al. 1998) expressed on the cell suface of endothelial cells and haematopoietic stem cells.
Key marker of canine hematopoietic progenitor cells.
Reported for use in CD34+ enrichment studies, (Goerner, M. et al. 2001) and (Horn, P. et al. 2004).
Applications
Suitable for use in Flow Cytometry and Western Blot. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Canine CD34 fusion protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™490.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes canine CD34.
