Mouse Anti-Granulocytes (Neutrophil Lineage) (MaxLight 550)
MaxLight™550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
Recognizes a 60kD antigen on porcine granulocytes of the neutrophil lineage, acting as a reliable tool for their analysis and isolation, without contamination from other cells.
Expression of the antigen recognized decreases from the immature promyelocytes, through myelocytes and metamyelocytes, to the mature neutrophils, thereby enabling the identification of neutrophil developmental stages. Furthermore, conjunction with other clones, allows for the discrimination and characterisation of different porcine granulocyte lineages and also their developmental stages: early myeloid precursors, immature neutrophils, mature neutrophils and mature eosinophils and basophils.
Has been shown as suitable for use on cytospins (Perez et al. 2007).
Applications
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation. Other applications not tested.
Recommended Dilution
Flow Cytometry: 1:25-1:200 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Porcine bone marrow haematopoietic cells (BMHC).
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™550.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes porcine Granulocytes.
