228153
Clone Type
MonoclonalHost
RatSource
MouseConjugate
APCIsotype
IgG2a,kClone Number
MEL-14Grade
PurifiedApplications
FCCrossreactivity
MoGene ID
20343Shipping Temp
Blue IceStorage Temp
4°C Do Not FreezeRat Anti-CD62L (L-Selectin, LECAM-1, LAM-1, Ly-22, Sell, Lnhr, Lyam-1) (APC)
CD62L, also known as L-selectin, is a type I transmembrane glycoprotein with lectin-like and epidermal growth factor-like domains. It binds to sialylated oligosaccharide determinants on high endothelial venules (HEV) in peripheral lymph nodes. In the mouse, CD62L is expressed on most thymocytes and on peripheral leukocytes, including B and T lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin family of cell adhesion molecules appears to be required for lymphocyte homing to peripheral lymph nodes and for leukocyte extravasation at sites of inflammation. Expression of CD62L on lymphocytes and neutrophils is rapidly down-regulated upon cell activation and the level of CD62L expression, along with other markers, distinguishes naive T cells from effector/memory T cells. The monoclonal antibody blocks in vitro binding of lymphocytes to peripheral lymph node HEV and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and the late stages of leukocyte rolling.
Applications
Suitable for use in Flow Cytometry. Other applications not tested.
Recommended Dilution
Flow Cytometry: 0.1ug/10e6 cells Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
Mouse B cell lymphoma 38C-13.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity
Recognizes mouse CD62L.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gallatin WM, Weissman IL, Butcher EC. A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature. 1983;304:30-4. (Immunogen, FC, IP, Block) 2. Reichert RA, Gallatin WM, Butcher EC, Weissman IL. A homing receptor-bearing cortical thymocyte subset: implications for thymus cell migration and the nature of cortisone-resistant thymocytes. Cell. 1984;38:89-99. (IHC-FS, FC) 3. Reichert RA, Jerabek L, Gallatin WM, Butcher EC, Weissman IL. Ontogeny of lymphocyte homing receptor expression in the mouse thymus. J Immunol. 1986;136:3535-42. (IHC-FS, FC) 4. Lewinsohn DM, Bargatze RF, Butcher EC. Leukocyte-endothelial cell recognition: evidence of a common molecular mechanism shared by neutrophils, lymphocytes, and other leukocytes. J Immunol. 1987;138:4313-21. (FC, IP, Block) 5. Seth A, Gote L, Nagarkatti M, Nagarkatti PS. T-cell-receptor-independent activation of cytolytic activity of cytotoxic T lymphocytes mediated through CD44 ad gp90MEL-14. Proc Natl Acad Sci USA. 1991;88:7877-81. (Cyto) 6. Takedachi M, Qu D, Ebisuno Y, Oohara H, Joachims ML, McGee ST, et al. CD73-generated adenosine restricts lymphocyte migration into draining lymph nodes. J Immunol. 2008;180:6288-96. (Block) 7. Diamant E, Keren Z, Melamed D. CD19 regulates positive selection and maturation in B lymphopoiesis: lack of CD19 imposes developmental arrest of immature B cells and consequential stimulation of receptor editing. Blood. 2005;105:3247-54. (FC) 8 .Duchesneau P, Gallagher E, Walcheck B, Waddell TK. Up-regulation of leukocyte CXCR4 expression by sulfatide: an L-selectin- dependent pathway on CD4+ T cells. Eur J Immunol. 2007;37:2949-60. (FC) 9. Bradfield PF, Scheiermann C, Nourshargh S, Ody C, Luscinskas FW, Rainger GE, et al. JAM-C regulates unidirectional monocyte transendothelial migration in inflammation. Blood. 2007;110:2545-55. (FC) 10 .Berge T, Grønningsæter IH, Lorvik KB, Abrahamsen G, Granum S, Sundvold-Gjerstad V, et al. SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice. PLoS One. 2012;7(10):e48239. (FC)USBio References
No references available