Technical Data

244681-ML490
Clone Type
Monoclonal
Host
Mouse
Conjugate
MaxLight™490
Isotype
IgG1,k
Clone Number
3F8
Grade
Purified
Applications
FLISA WB
Accession #
AAH11393
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
Mouse Anti-CHN1 (Chimerin (chimaerin) 1, ARHGAP2, CHN, DURS2, RHOGAP2) (MaxLight 490)
Chimerin (chimaerin) 1, ARHGAP2, CHN, DURS2, RHOGAP2

MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000.

This gene encodes GTPase-activating protein for p21-rac and a phorbol ester receptor. It plays an important role in ocular motor axon pathfinding. Heterozygous missense mutations in this gene cause Duane's retraction syndrome 2 (DURS2). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
QTRNFRLYYDGKHFVGEKRFESIHDLVTDGLITLYIETKAAEYIAKMTINPIYEHVGYTTLNREPAYKKHMPVLKETHDERDSTGQDGVSEKRLTSLVRRATLKENEQIP
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
CHN1 (AAH11393, 91aa-200aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Purity
Purified
Specificity
Recognizes CHN1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Tsc2-Rheb signaling regulates EphA-mediated axon guidance.Nie D, Di Nardo A, Han JM, Baharanyi H, Kramvis I, Huynh T, Dabora S, Codeluppi S, Pandolfi PP, Pasquale EB, Sahin M.Nat Neurosci. 2010 Jan 10. [Epub ahead of print]
USBio References
No references available
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