Technical Data

246467
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3C2
Grade
Purified
Applications
E WB
Crossreactivity
Hu
Accession #
NP_002037
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase, G3PD, GAPD, MGC88685)
Glyceraldehyde-3-Phosphate Dehydrogenase, G3PD, GAPD, MGC88685

The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome. [provided by RefSeq

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
GKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
GAPDH (NP_002037, 226aa-335aa) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Purified
Specificity
Recognizes human GAPDH.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1.Reticulon 3 interacts with NS4B of the hepatitis C virus and negatively regulates viral replication by disrupting NS4B self-interaction.Wu MJ, Ke PY, Hsu JT, Yeh CT, Horng JTCell Microbiol. 2014 Jun 4. doi: 10.1111/cmi.12318. 2.Receptor for Activated Protein Kinase C: Requirement for Efficient MicroRNA Function and Reduced Expression in Hepatocellular Carcinoma. Otsuka M, Takata A, Yoshikawa T, Kojima K, Kishikawa T, Shibata C, Takekawa M, Yoshida H, Omata M, Koike K.PLoS One. 2011;6(9):e24359. Epub 2011 Sep 15. 3.Expression and roles of Slit/Robo in human ovarian cancer. Dai CF, Jiang YZ, Li Y, Wang K, Liu PS, Patankar MS, Zheng J.Histochem Cell Biol. 2011 Apr 5. [Epub ahead of print] 4. Anti-angiogenic effect of triterpenoidal saponins from Polygala senega. Arai M, Hayashi A, Sobou M, Ishida S, Kawachi T, Kotoku N, Kobayashi M.J Nat Med. 2010 Nov 2. [Epub ahead of print] 5. The Effect of Allelic Variation in Aldo-Keto Reductase 1C2 on the In Vitro Metabolism of Dihydrotestosterone.|Takahashi RH, Grigliatti TA, Reid RE, Riggs KW.J Pharmacol Exp Ther. 2009 Jun;329(3):1032-9. Epub 2009 Mar 3.
USBio References
No references available
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