Mouse Anti-ID4 (Inhibitor of DNA Binding 4, Dominant Negative Helix-loop-Helix Protein, IDB4, bHLHb27) (Biotin)
Inhibitor of DNA Binding 4, Dominant Negative Helix-loop-Helix Protein, IDB4, bHLHb27
Transcription factors containing a basic helix-loop-helix (bHLH) motif regulate expression of tissue-specific genes in a number of mammalian and insect systems. DNA-binding activity of the bHLH proteins is dependent on formation of homo- and/or heterodimers. Dominant-negative HLH proteins encoded by Id-related genes, such as ID4, also contain the HLH-dimerization domain but lack the DNA-binding basic domain. Consequently, Id proteins inhibit binding to DNA and transcriptional transactivation by heterodimerization with bHLH proteins (Pagliuca et al., 1995 [PubMed 7665172]).[supplied by OMIM
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
AA Sequence
MKAVSPVRPSGRKAPSGCGGGELALRCLAEHGHSLGGSMouse monoclonal antibody raised against a full-length recombinant ID4.Mouse monoclonal antibody raised against a full-length recombinant ID4.Mouse monoclonal antibody raised against a full-length recombinant ID4.ARCKAAEMouse monoclonal antibody raised against a full-length recombinant ID4.DEPALCLQCDMNDCYSRLRRLVPTIPPNKKVSKVEILQHVIDYILDLQLALETHPALLRQPPPPAPPHHPAGTCPAAPPRTPLTALNTDPAGAVNKQGDSILCR
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
ID4 (NP_001537.1, 1aa-161aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity
Recognizes ID4.
