Technical Data

251590
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1,k
Clone Number
3G8
Grade
Purified
Applications
E IF IHC WB
Crossreactivity
Hu
Accession #
AAH01263
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-SGK (Serum/Glucocorticoid Regulated Kinase 1, SGK)
Serum/Glucocorticoid Regulated Kinase 1, SGK

SGK, a SGK type protein kinase, is a multi-functional kinase that is phosphorylated and activated through a 3-phosphoinositide-dependent protein kinase 1 (PDK1) signaling pathway. The protein has been shown to activate certain potassium, sodium and chloride channels which function in cell survival, neuronal excitability and renal sodium excretion. SGK transcription can be stimulated by glucocorticoids, aldosterone, TGF-beta1, serum, increase of intracellular Ca2+ and phorbolesters, and can be downregulated by cAMP. At least 2 mRNA variants have been reported. The SGK1 gene has been mapped to chromosome 6q23, a region frequently affected by deletion in various human neoplasms, and has been shown to be upregulated in lung fibrosis, diabetic nephropathy and chronic viral hepatitis.

Applications
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Immunofluorescence: 10ug/ml Immunohistochemistry (FFPE): 5ug/ml Requires HIER using sodium citrate buffer pH 6.0 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa1-90 from human SGK with GST tag. MW of the GST tag alone is 26kD.
Form
Supplied as a liquid in PBS, pH 7.4.
Purity
Purified
Specificity
Recognizes human SGK.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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