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253943 Mouse Anti-Beta Amyloid (A4, AAA, ABETA, ABPP, AD1, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), Alzheimer Disease Amyloid Protein, Amyloid beta (A4) precursor Protein, Amyloid beta A4 Protein, Amyloid intracellular Domain 50, Amyloid intracellular Domain 57, Amyloid intracellular Domain 59, APP, APPI, beta-Amyloid peptide, Beta-Amyloid Protein 40, Beta-Amyloid Protein 42, Beta-APP40, Beta-APP42, C31, C80, C83, C99, Cerebral vascular Amyloid peptide, CTFgamma, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), peptidase nexin-II, PN-II, PN2, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta) (HRP)

Specifications
References
Clone Type
Monoclonal
Host
Mouse
Source
Human
Conjugate
HRP
Isotype
IgG1
Clone Number
DE2B4
Grade
Affinity Purified
Applications
IC IHC IP
Crossreactivity
Bo Hu Po
Shipping Temp
Blue Ice
Storage Temp
-20°C
A4, AAA, ABETA, ABPP, AD1, AICD-50, AICD-57, AICD-59, AID(50), AID(57), AID(59), Alzheimer disease amyloid protein, amyloid beta (A4) precursor protein, Amyloid beta A4 protein, Amyloid intracellular domain 50, Amyloid intracellular domain 57, Amyloid intracellular domain 59, APP, APPI, beta-amyloid peptide, Beta-amyloid protein 40, Beta-amyloid protein 42, Beta-APP40, Beta-APP42, C31, C80, C83, C99, Cerebral vascular amyloid peptide, CTFgamma, CVAP, Gamma-CTF(50), Gamma-CTF(57), Gamma-CTF(59), Gamma-secretase C-terminal fragment 50, Gamma-secretase C-terminal fragment 57, Gamma-secretase C-terminal fragment 59, N-APP, P3(40), P3(42), peptidase nexin-II, PN-II, PN2, PreA4, Protease nexin-II, S-APP-alpha, S-APP-beta, Soluble APP-alpha, Soluble APP-beta

DE2B4 has been used to stain amyloid plaques in the brain.

Applications
Suitable for use in Immunoprecipitation, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to residues 1-16 of the human amyloid beta protein
Form
Supplied as a liquid in PBS, 0.01% Thiomersal. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Beta Amyloid. Species Crossreactivity: Human, Porcine, Bovine. Does not cross react with Mouse or Rat.
References
1. Corona C et. al. Dietary zinc supplementation of 3xTg-AD mice increases BDNF levels and prevents cognitive deficits as well as mitochondrial dysfunction. Cell Death Dis. 1(10): e91 (2010).
USBio References
No references available
Conjugates
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