Technical Data

253966
Clone Type
Monoclonal
Host
Rat
Source
Human
Isotype
IgG2b
Clone Number
7E7
Grade
Supernatant
Applications
FC IC IF IHC
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-CCR9 (Chemokine (C-C Motif) Receptor 9 isoform A, Chemokine (C-C Motif) Receptor 9 isoform B, CC chemokine Receptor D6, C C chemokine Receptor Type 9, C C CKR 9, CC ChemokineReceptor CCR10, CC CKR 9, CCBP2, CCCKR9, CCR 9, CCR9, CDw199, CDw199 antigen, Chemokine (C C Motif) Receptor 9, isoform CRA_a, Chemokine C C Motif Receptor 9, Chemokine C C Receptor 10, Chemokine CC Motif Receptor9, Chemokine Receptor CCR 9, Chemokine Receptor CCR9, Chemokine-Binding Protein D6, Cmkbr10, CMKBR9, G Protein coupled Receptor 28, GPR 28, GPR 9 6, GPR28, GPR96, OTTHUMP00000164653, OTTHUMP00000164654)
Chemokine (C-C motif) receptor 9 isoform A, Chemokine (C-C motif) receptor 9 isoform B, CC chemokine receptor D6, C C chemokine receptor type 9, C C CKR 9, CC Chemokinereceptor CCR10, CC CKR 9, CCBP2, CCCKR9, CCR 9, CCR9, CDw199, CDw199 antigen, Chemokine (C C motif) receptor 9, isoform CRA_a, Chemokine C C motif receptor 9, Chemokine C C receptor 10, Chemokine CC Motif Receptor9, Chemokine receptor CCR 9, Chemokine receptor CCR9, Chemokine-binding protein D6, Cmkbr10, CMKBR9, G protein coupled receptor 28, GPR 28, GPR 9 6, GPR28, GPR96, OTTHUMP00000164653, OTTHUMP00000164654

CCR9, a chemokine receptor, is expressed in thymocytes and T lymphocytes from the small intestine and colon but not in lymphocytes from tonsils, lung, inflamed liver, normal or inflamed skin, inflamed synovium and synovial fluid, breast milk, and seminal fluid (Kunkel et al., 2000). CCR9 is not detected in natural killer cells, monocytes, eosinophils, basophils, and neutrophils. CCR9 binds TECK (thymus-expressed chemokine), a CC chemokine expressed in thymus and small intestine.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide: PTDFTSPIPNMADDYGSEST, corresponding to N-terminal aa3-22 of Mouse CCR9. Species sequence homology: Human
Form
Supplied as a liquid in RPMI 1640, 5 % FCS, 0.09% sodium azide.
Purity
Supernatant
Specificity
Recognizes CCR9. Species Crossreactivity: Mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Pabst O  et al. Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine. J Exp Med 199:411-6 (2004) PMID: 14744993.
USBio References
No references available
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