Mouse Anti-CD11/CD18 (Integrin alpha (L,M,X)/beta 2) (FITC)
Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits which mediate cell-cell and cell-extracellular matrix attachments. Integrin beta2 (CD18) associates with integrin alpha L (CD11a) to form the leukocyte function-associated antigen-1 (LFA-1) with integrin alpha M (CD11b) to form Complement Receptor 3 (CR3) and with integrin alpha X (CD11c) to form Complement Receptor 4 (CR4). Each integrin receptor can bind various Intercellular adhesion molecules (ICAMs). Aberrant integrin expression has been found in many epithelial tumors. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 Ag.14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fibronectin-purified human monocytes
Form
Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein A affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human integrin alpha L,M,X/Beta 2 (CD11/CD18).
