Mouse Anti-CRISPR (Cas9, CRISPR-Associated Endonuclease Cas9/Csn1, CRISPR-Cas9/Csn1, Csn1, SpyCas9)
Cas9, CRISPR-associated endonuclease Cas9/Csn1, CRISPR-Cas9/Csn1, csn1, SpyCas9
Clustered regularly interspaced short palindromic repeat (CRISPR) and associated CRISPR-associated nuclease (cas) proteins comprise the CRISPR/cas systems that are responsible for adaptive immunity in many bacteria and archaea. Three types of CRISPR/cas systems have been identified to date, which have been designated type I, II and III. Cas9 is an RNA guided DNA nuclease associated with the type II CRISPR/Cas adaptive immune system. In prokaryotes, the CRISPR/Cas9 system provides protection against viruses and plasmids by targeting foreign nucleic acids with short complementary single stranded RNA that localizes the Cas9 nuclease to the target DNA sequence, introducing double strand breaks at specific locations within the genomic DNA.
In recent years, scientists have recognized the importance of the CRISPR/cas system as a tool for genomic editing and engineering. In particular, the type II CRISPR/cas9 system derived from Streptococcus pyogenes has been adapted and utilized as the genome engineering tool of choice. The CRISPR/Cas9 system has the potential to transform developmental biology by providing a simple, efficient method to precisely manipulate the genome of virtually any developing organism
Applications
Suitable for use in Immunofluorescence/Immunocytochemistry and Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-1ug/ml Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein within the N-terminal region of Streptococcus pyogene Cas9
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography from supernatant.
Specificity
Recognizes Cas9 and dCas9 based on the antigen design. Species Crossreactivity: Streptococcus pyogene
