Mouse Anti-EGFR (Avian Erythroblastic Leukemia viral (v erb b) Oncogene Homolog, Avian Erythroblastic Leukemia viral (verbb) Oncogene Homolog, Cell Growth inhibiting Protein 40, Cell proliferation inducing Protein 61, EGF R, EGFR, EGFR, EGFR_HUMAN, Epidermal Growth Factor Receptor (avian Erythroblastic Leukemia viral (v erb b) Oncogene Homolog), Epidermal Growth Factor Receptor (Erythroblastic Leukemia viral (v erb b) Oncogene Homolog avian), Epidermal Growth Factor Receptor, erbb 1, Erbb, Erbb1, ERBB1, Errp, HER1, mENA, Oncogene ERBB, PIG61, Proto-Oncogene c-ErbB-1, Receptor Tyrosine Protein Kinase ErbB 1, Receptor Tyrosine Protein Kinase ErbB1, Receptor Tyrosine-Protein Kinase ErbB-1, Urogastrone, wa2, Wa5)
Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Avian erythroblastic leukemia viral (verbb) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, EGF R, EGFR, EGFR, EGFR_HUMAN, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian), Epidermal growth factor receptor, erbb 1, Erbb, Erbb1, ERBB1, Errp, HER1, mENA, Oncogene ERBB, PIG61, Proto-oncogene c-ErbB-1, Receptor tyrosine protein kinase ErbB 1, Receptor tyrosine protein kinase ErbB1, Receptor tyrosine-protein kinase ErbB-1, Urogastrone, wa2, Wa5
EGFR is a transmembrane glycoprotein that is a member of a family of protein tyrosine kinases crucial in maintaining a normal balance in cell growth and development. A prototype member of the type 1 receptor tyrosine kinases, EGFR is encoded by the cellular oncogene cerbB1. EGFR has an extracellular ligand binding domain, a single transmembrane region, and cytoplasmic domain which is composed of a tyrosine kinase domain and a carboxy terminal domain. The carboxy terminal domain contains at least four tyrosine autophosphorylation sites. Increased production or activation of EGFR has been associated with poor prognosis in a variety of tumors. EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.
Applications
Suitable for use in ELISA, Immunoprecipitation, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
NS1 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide (residues 985-996 of human EGFR) coupled to keyhole limpet hemocyanin
Form
Supplied as a liquid in PBS, 0.02% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes Epidermal Growth Factor Receptor Species Crossreactivity: Human
