Mouse Anti-HDAC3 (RPD3 2, HD 3, HD3, HDAC 3, HDAC3, HDAC3_HUMAN, Histone Deacetylase 3 (HD3) (RPD3-2), Histone Deacetylase 3, RPD 3, RPD3, RPD3-2, SMAP 45, SMAP45)
RPD3 2, HD 3, HD3, HDAC 3, HDAC3, HDAC3_HUMAN, Histone deacetylase 3 (HD3) (RPD3-2), histone deacetylase 3, RPD 3, RPD3, RPD3-2, SMAP 45, SMAP45
Histone deacetylase 3 (HDAC3), which is a transcriptional regulator of the histone deacetylase family, subfamily 1. HDAC3 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation plays a role in epigenetic repression and transcriptional regulation, and cell cycle progression.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:400-1:800 Optimal dilutions to be determined by the researcher.
Hybridoma
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Human C-terminal peptide (identical in mouse). Sequence: aa411-428 (NEFYDGDHDNDKESDVEI)
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes HDAC3. Species Crossreactivity: Human, Mouse
