Technical Data

254115
Clone Type
Monoclonal
Host
Mouse
Source
Human
Isotype
IgG1
Clone Number
3G6
Grade
Affinity Purified
Applications
ChIP E FC IC IF IHC IP WB
Crossreactivity
Hu Mk Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-hnRNP U (Heterogeneous Nuclear Ribonucleoprotein U, hnRNP U Protein, HNRNPU, hnRNPUProtein, HNRPU, p120, p120 Nuclear Protein, pp120, SAF A, SAF-A, SAFA, Scaffold attachment Factor A, U21.1)
Heterogeneous nuclear ribonucleoprotein U, hnRNP U protein, HNRNPU, hnRNPUprotein, HNRPU, p120, p120 nuclear protein, pp120, SAF A, SAF-A, SAFA, Scaffold attachment factor A, U21.1

The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties hnRNP U is thought to be involved in the packaging of hnRNA into large ribonucleoprotein complexes. During apoptosis this protein is cleaved in a caspase-dependent way.

Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, ChIP. Other applications not tested.
Recommended Dilution
Western Blot: Use at an assay dependent dilution. Detects a band of ~97kD (predicted molecular weight: 120kD). Immunocytochemistry/Immunofluorescence: 1:100 Flow Cytometry: 1ug for 10e6 cells Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
RNPs eluted from an oligo (dt) cellulose column
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes hnRNP U. Species Crossreactivity: Mouse, Human, Monkey. Does not react with Chicken, Lizard

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Ma N  et al. The nuclear scaffold protein SAF-A is required for kinetochore-microtubule attachment and contributes to the targeting of Aurora-A to mitotic spindles. J Cell Sci 124:394-404 (2011). 2. Tserel L  et al. MicroRNA Expression Profiles of Human Blood Monocyte-derived Dendritic Cells and Macrophages Reveal miR-511 as Putative Positive Regulator of Toll-like Receptor 4. J Biol Chem 286:26487-95 (2011). WB ; Human. 3. Raju CS  et al. In neurons, activity-dependent association of dendritically transported mRNA transcripts with the transacting factor CBF-A is mediated by A2RE/RTS elements. Mol Biol Cell 22:1864-77 (2011). WB ; Mouse. 4. Pare JM  et al. MicroRNA-binding is required for recruitment of human Argonaute 2 to stress granules and P-bodies. Biochem Biophys Res Commun : (2011). WB ; Human. 5. Hadian K  et al. Identification of a heterogeneous nuclear ribonucleoprotein-recognition region in the HIV Rev protein. J Biol Chem 284:33384-91 (2009). WB ; Human. 6. Pacheco A et al. A novel role for Gemin5 in mRNA translation. Nucleic Acids Res. 2009 Feb; 37(2):582-90. doi: 10.1093/nar/gkn979. Epub 2008 Dec 9. WB, IP ; Human PMID: 19066202. 7. Uchimura Y et al. Involvement of SUMO modification in MBD1- and MCAF1-mediated heterochromatin formation. J Biol Chem. 2006 Aug 11; 281(32):23180-90. Epub 2006 Jun 5. ICC/IF ; Human PMID: 16757475. 8. HnRNP L represses exon splicing via a regulated exonic splicing silencer. Rothrock CR et al. EMBO J. 2005 Aug 3; 24(15):2792-802. Epub 2005 Jul 7. 9. Ahmad N et al. Kruppel-like factor 2 transcriptional regulation involves heterogeneous nuclear ribonucleoproteins and acetyltransferases. Biochemistry. 2005 Apr 26; 44(16):6276-85. ChIP, EMSA ; Mouse PMID: 15835916. 10. Dreyfuss G  et al. Characterization of heterogeneous nuclear RNA-protein complexes in vivo with monoclonal antibodies. Mol Cell Biol 4:1104-14 (1984).
USBio References
No references available
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