Mouse Anti-Lamin A/C (70kD Lamin, Cardiomyopathy Dilated 1A (Autosomal Dominant), CDCD1, CDDC, Charcot Marie Tooth Disease Axonal Type 2B1, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, Lamin A/C, Lamin A/C Isoform 1Precursor, Lamin A/C Isoform 2, Lamin A/C Isoform 3, LDP1, LFP, LGMD1B, Limb girdle muscular dystrophy 1B (Autosomal Dominant), LMN 1, LMN A, LMN C, LMN1, LMNA, LMNC, NY REN 32 antigen, PRO1, Progeria 1 (Hutchinson Gilford Type), Renal carcinoma antigen NY REN 32, Renalcarcinoma antigen NYREN32)
Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished ie A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing ie Lamin A Lamin C and Lamin Adel 10 while the B-type Lamins include two proteins arising from two distinct genes ie Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders including Emery-Dreifuss muscular dystrophy dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition the expression of A-type Lamins coincides with cell differentiation and as A-type. Lamins specifically interact with chromatin a role in the regulation of differential gene expression has been suggested for A-type Lamins.
Applications
Suitable for use in Immunofluorescence, Western Blot, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Immunocytochemistry/Immunofluorescence: 1:10 Western Blot: 1:50. Detects a band of ~70kD (Lamin A) and 60kD (Lamin C). Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Full length recombinant protein corresponding to Lamin A+C, bacterially expressed.
Form
Supplied as a liquid in 0.09% sodium azide.
Specificity
Recognizes un-phosphorylated form of lamin A/C and the epitope is mapped to the N-terminal head domain of both proteins. Reacts with mouse myoblasts c2c12 in Western blot. Other cell/tissue types have not been tested. Species Crossreactivity: Mouse, Human
