Mouse Anti-XPF (DNA Excision Repair Protein ERCC-4, DNA Repair Endonuclease XPF, DNA Repair Protein Complementing XP-F cells, ERCC11, ERCC4, Excision Repair cross-Complementing rodent Repair deficiency, Complementation group 4, Excision-Repair, Complementing Defective, in Chinese hamster, RAD1, Xeroderma Pigmentosum group F-Complementing Protein, xeroderma Pigmentosum, Complementation group F, XPF)
DNA excision repair protein ERCC-4, DNA repair endonuclease XPF, DNA repair protein complementing XP-F cells, ERCC11, ERCC4, excision repair cross-complementing rodent repair deficiency, complementation group 4, excision-repair, complementing defective, in Chinese hamster, RAD1, Xeroderma pigmentosum group F-complementing protein, xeroderma pigmentosum, complementation group F, XPF
DNA repair endonuclease XPF, which is also known as ERCC4. Nucleotide excision repair (NER) is a DNA repair pathway that removes lesions induced by a variety of agents such as UV irradiation. XPF forms a heterodimer with ERCC1, and functions as a DNA repair endonuclease for the 5-prime incision during DNA repair. XPF is involved in homologous recombination that assists in removing interstrand cross-link.
Applications
Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested.
Recommended Dilution
Optimal dilutions to be determined by the researcher.
Hybridoma
Sp2/0 Ag.14 myeloma cells with spleen cells from Balb/c mice.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein His-tagged fragment of human XPF protein produced in E. coli
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes DNA repair endonuclease XPF. Species Crossreactivity: Human
