Technical Data

298043
Clone Type
Monoclonal
Host
Rat
Source
Mouse
Isotype
IgG2a
Clone Number
C9-6-25-5
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rat Anti-C9 (Complement Component C9)

The monoclonal antibody C9 recognizes mouse complement component C9. The complement system is an ancient proinflammatory and microbial destruction system, that may be considered part of both the innate and adaptive immune systems. It consists of the classical, alternative, and lectin-binding pathways. Each pathway is triggered in a distinct manner, yet all deposit C3 fragments on a target and engage a common terminal sequence called TCC or the "membrane attack complex" (MAC). In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding of the different subunits. TCC is composed of a complex of four complement proteins (C5b, C6, C7, and C8) which bind to the outer surface of the target plasma membrane, and many copies of a fifth protein (C9) that hookup to one another, forming a ring in the membrane. The ring structure formed by C9 is a pore in the membrane that allows free diffusion of molecules in and out of the cell. If enough pores form, the cell is no longer able to survive. The membrane attack complex is initiated when the complement protein, C5 convertase, cleaves C5 into C5a and C5b. Binding of C6 facilitates binding of C7 which alters the conformation of the complex. After binding of C8, a variable number of C9 molecules associate with the C5b678 complex, together constituting TCC. The formation of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:50 using reduced sample treatment and SDS-PAGE. The band size is 72kD. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse C9 peptide
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse C9.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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