Technical Data

298061
Clone Type
Monoclonal
Host
Mouse
Source
Mouse
Conjugate
FITC
Isotype
IgG1
Clone Number
1A2
Grade
Affinity Purified
Applications
E IHC WB
Crossreactivity
Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Mouse Anti-Complement Factor H (Factor H, CFH) (FITC)

Monoclonal antibody Factor H recognizes mouse complement factor H (CFH). CFH is the first regulatory protein of the alternative pathway of the complement system. There are three pathways of complement activation; classical, alternative, and lectin activation pathway. These pathways converge to form C3/C5 convertases that generate C3a, C3b,C5a, and C5b, each with substantial biologic activity. Complement regulators are necessary to prevent the injudicious production of these mediators and potential injury to self-tissue. The plasma proteins CFH, C4-binding protein and the cell membrane proteins complement receptor 1 (CR1; CD35), decay-accelerating factor (CD55), and membrane co-factor protein (CD46) all are members of the regulators of complement activation family. These proteins have natural affinity for C3b and/or C4b, which confers on them the ability to accelerate the intrinsic decay of C3/C5 convertases and/or act as co-factor for the cleavage and inactivation (i) of C3b and C4b by complement factor I (CFI). Genetic human analyses reveals a clear association of CFH with different human diseases. These include diseases of the kidney, the atypical form of Hemolytic Uremic Syndrome (aHUS) and membranoproliferative glomerulonephritis (MPGN). Furthermore, CFH is associated with age-related macular degeneration (AMD), a disease of the eye.

Applications
Suitable for use in ELISA, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:50 Immunohistochemistry(Frozen): 1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Mouse factor H-human IgG fusion protein
Form
Supplied as a liquid in PBS, 0.1% BSA, 0.02% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes mouse Factor H. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
No references available
USBio References
No references available
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