Technical Data

301371
Clone Type
Polyclonal
Host
Goat
Isotype
IgG
Grade
Serum
Applications
WB
Shipping Temp
Blue Ice
Storage Temp
-20°C
Goat Anti-HNE (4-Hydroxy Nonenal) (Not for Export EU)

4-Hydroxynonenal (HNE) is a highly reactive, cytotoxic aldehyde. HNE can efficiently react with sulfhydryl groups or histidine and lysine groups of proteins to form stable HNE-protein adducts, in addition to phospholipids and nucleic acids. It is through this HNE-modification of biomolecules that HNE is thought to exert its cytotoxic effects.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: >1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
4-Hydroxynonenal protein
Form
Supplied as a liquid.
Purity
Serum
Specificity
Recognizes 4-Hydroxynonenal.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Philip L. Davies, Nicola C. Maxwell, Sailesh Kotecha, O. Brad Spiller. Monoclonal anti-neutrophil elastase antibody characterisation: Ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granule. Journal of Immunological Methods. Volume: 336, Issue: 2, Page: 175-182. DOI: 10.1016/j.jim.2008.04.010
USBio References
No references available
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