Rabbit Anti-NPFF2 (Neuropeptide FF2, NPFF-2, NPFF 2) (HRP)
The central nervous system neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance as well as other physiological functions. Two G protein-coupled receptors, NPFF1 and NPFF2, were isolated from the human central nervous system.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
N-terminal sequence (MNEKWDTNSSENWHPI) and C-terminal sequence (ELVMEELKETTNSSEI) of human NPFF2.
Form
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human NPFF2.
