Rabbit Anti-PHF2 (PHD Finger Protein 2, PHF-2, PHF 2) (APC)
Plant homeodomain (PHD) finger protein 2 (PHF2) is a putative transcription factor. PHF2 contains a zinc finger-like PHD domain that is distinct from other classes of zinc finger motifs and is often found in proteins that influence chromatin structure. It also contains a Jumonji C (JmjC) domain, which may play a role in histone demethylation. The PHF2 gene is ubiquitously expressed in adult mouse tissues; however, the majority of PHF2 expression in the mouse embryo occurs in the neural tube and root ganglia. PHF2 mutations have been associated with both early- and late-onset breast carcinoma.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
Immunogen
Residues aa70-82 [KHGPGPTPDVKRVC] of the 120kD PHF protein.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes PHF2.
