Mouse Anti-CDC-5 (CDC5, MSD2)
Polo-like kinases (PLKs) are evolutionarily conserved kinases essential for cell division, proliferation, and development. These kinases are characterized by the presence of a C-terminal phosphopeptide- interaction domain, the polo-box domain (PBD). Cdc5 (Msd2) is a cell cycle serine/threonine-protein kinase of Saccharomyces cerevisiae involved in mitotic exit. Recently, a novel function for Cdc5 as an important regulator of cellular ploidy has been reported.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:250-1:500 Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to full length Saccharomyces cerevisiae CDC-5.
Form
Supplied as a lyophilized powder in PBS, pH 7.4. No preservative added. Reconstitute with 100ul sterile ddH2O.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes full length Saccharomyces cerevisiae Cdc5
