Rabbit Anti-ERVK-7 (Endogenous Retrovirus group K Member 7 Env Polyprotein, Envelope Polyprotein, HERV-K(III) Envelope Protein, HERV-K102 Envelope Protein, HERV-K_1q22 provirus ancestral Env Polyprotein, Surface Protein, SU, Transmembrane Protein, TM, ERVK-7)
Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane (By similarity).
Applications
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilutions
Western Blot: 1:1000-1:2000 Immunohistochemistry (FFPE): 1:25 Requires HIER using sodium citrate buffer pH6.0 prior to staining. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH conjugated synthetic peptide corresponding to aa96-128 from human ERVK-7.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by ammonium sulfate precipitation.
Specificity
Recognizes human ERVK-7.
