Technical Data

302513
Clone Type
Polyclonal
Host
Rabbit
Source
Mouse
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Mo
Gene ID
16468
Gene #
Jarid2
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Jarid2 (Protein Jumonji, Jumonji/ARID Domain-Containing Protein 2, Jarid2, Jmj)

Regulator of histone methyltransferase complexes that plays an essential role in embryonic development, including heart and liver development, neural tube fusion process and hematopoiesis. Acts by modulating histone methyltransferase activity and promoting the recruitment of histone methyltransferase complexes to their target genes. Binds DNA and mediates the recruitment of the PRC2 complex to target genes in embryonic stem cells. Does not have histone demethylase activity but regulates activity of various histone methyltransferase complexes. In embryonic stem cells, it associates with the PRC2 complex and inhibits trimethylation of 'Lys-27' of histone H3 (H3K27me3) by the PRC2 complex, thereby playing a key role in differentiation of embryonic stem cells and normal development. In cardiac cells, it is required to repress expression of cyclin-D1 (CCND1) by activating methylation of 'Lys-9' of histone H3 (H3K9me) by the GLP1/EHMT1 and G9a/EHMT2 histone methyltransferases. Also acts as a transcriptional repressor of ANF via its interaction with GATA4 and NKX2-5. Participates in the negative regulation of cell proliferation signaling.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:2000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH conjugated synthetic peptide corresponding to aa435-468 at the Central region of Mouse Jarid2.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Jarid2. Species Crossreactivity: mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Takeuchi T.,et al.Genes Dev. 9:1211-1222(1995).|Carninci P.,et al.Science 309:1559-1563(2005).|Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.|Motoyama J.,et al.Mech. Dev. 66:27-37(1997).|Takeuchi T.,et al.Mech. Dev. 86:29-38(1999).|
USBio References
No references available
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