Rabbit Anti-Nkx2-5 (Homeobox Protein Nkx-25, Cardiac-specific Homeobox, Homeobox Protein CSX, Homeobox Protein NK-2 Homolog E, Nkx2-5, Csx, Nkx-25, Nkx2e)
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4. It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B. Together with PBX1, it is required for spleen development through a mechanism that involves CDKN2B repression.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
KLH conjugated synthetic peptide corresponding to aa192-235 at the Central region of mouse Nkx2-5. Species sequence homology: rat
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Purity
Purified by Protein A affinity chromatography.
Specificity
Recognizes mouse Nkx2-5. Species Crossreactivity: mouse
