Rabbit Anti-CD63 (Lysosomal Associated Membrane Protein 3, CD 63, CD63 Antigen (Melanoma 1 Antigen), CD63 Antigen, CD63 Antigen Melanoma 1 Antigen, CD63 Molecule, CD63_HUMAN, Gp55, Granulophysin, LAMP 3, LAMP-3, LAMP3, LIMP, Lysosomal-associated Membrane Protein 3, Lysosome Associated Membrane Glycoprotein 3, Mast Cell Antigen AD1, ME491, Melanoma 1 Antigen, Melanoma Associated Antigen ME491, Melanoma Associated Antigen MLA1, Melanoma-associated Antigen ME491, MLA 1, MLA1, NGA, Ocular Melanoma Associated Antigen, Ocular Melanoma-associated Antigen, OMA81H, PTLGP40, Tetraspanin 30, Tetraspanin-30, Tspan 30, Tspan-30, TSPAN30)
CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human CD63 expressed in E. coli. (Position: E97-M238). Species sequence homology: mouse (74%) and rat (73%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CD63.
