Rabbit Anti-CREB (Active Transcription Factor CREB, cAMP Response Element Binding Protein 1, cAMP Response Element Binding Protein, cAMP Responsive Element Binding Protein 1, cAMP-responsive Element-binding Protein 1, CREB 1, CREB-1, CREB1, Cyclic AMP-responsive Element-binding Protein 1, Transactivator Protein)
CREB1, also known as cAMP responsive element binding protein 1, is a protein that in humans is encoded by the CREB1 gene. It is mapped to 2q33.3. This protein binds the cAMP response element, a DNA nucleotide sequence present in many viral and cellular promoters. The binding of CREB1 stimulates transcription. This protein is a CREB transcription factor that is a member of the leucine zipper family of DNA-binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human CREB expressed in E. coli. (Position: M1-T177). Species sequence homology: mouse (99%) and rat (98%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human CREB. Species Crossreactivity: rat
