Technical Data

303320
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IC IHC WB
Crossreactivity
Hu Mo Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Galectin 1 (14kD Laminin-binding Protein, 14kD Lectin, Beta Galactoside Binding Lectin, Beta Galactoside Binding Lectin L 14 I, Beta Galactoside Binding Protein, Beta-galactoside-binding Lectin L-14-I, Gal 1, Gal-1, GAL1, Galaptin, Galbp, Galectin, Galectin-1, Galectin1, GBP, HBL, HLBP14, HPL, L 14.5, L-14.5, L14, Lactose Binding Lectin 1, Lactose-binding Lectin 1, Lect14, Lectin Galactoside Binding Soluble 1, Lectin Galactoside-binding Soluble 1, LEG1_HUMAN, LGALS 1, LGALS1, Lgals1 Lectin Galactose Binding Soluble 1, MAPK Activating Protein MP12, Putative MAPK Activating Protein MP12, Putative MAPK-activating Protein PM12, S Lac Lectin 1, S-Lac Lectin 1)

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Galectin-1 expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. It is thought to play a role in creating immune tolerance in pregnancy. It has been found that Galectin-1-mediated production of IL6 may assist in augmenting the innate immune response against NiV. Galectin-1 may also be a significant factor that augments the efficiency of the HIV-1 infection process.

Applications
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions. Immunohistochemistry (frozen): 0.5-1ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Immunocytochemistry: 0.5-1ug/ml Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human Galectin 1 expressed in E. coli. (Position: A2-D135). Species sequence homology: mouse (88%) and rat (90%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Galectin 1. Species Crossreactivity: mouse and rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Das, K., Lin, Y., Widen, E., Zhang, Y., Scherer, P. E. Chromosomal localization, expression pattern, and promoter analysis of the mouse gene encoding adipocyte-specific secretory protein Acrp30. Biochem. Biophys. Res. Commun. 280: 1120-1129, 2001. 2. Schaffler, A., Orso, E., Palitzsch, K.-D., Buchler, C., Drobnik, W., Furst, A., Scholmerich, J., Schmitz, G. The human apM-1, an adipocyte-specific gene linked to the family of TNF's and to genes expressed in activated T cells, is mapped to chromosome 1q21.3-q23, a susceptibility locus identified for familial combined hyperlipidaemia (FCH). Biochem. Biophys. Res. Commun. 260: 416-425, 1999.
USBio References
No references available
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