303330
Clone Type
PolyclonalHost
RabbitSource
HumanIsotype
IgGGrade
Affinity PurifiedApplications
IHC WBCrossreactivity
HuShipping Temp
Blue IceStorage Temp
-20°CRabbit Anti-GFRA1 (GDNF Family Receptor Alpha 1, GDNF R, GDNF RA, GDNF Receptor alpha, GDNFR alpha 1, GDNFR alpha, GDNFR, GDNFRA, GDNFRalpha, GFR alpha 1, GFR alpha1, GFR-Alpha-1, GFRA 1, GFRalpha1, Glial Cell Line Derived Neurotrophic Factor Receptor Alpha, Glial Cell Line-derived Neurotrophic Factor Receptor alpha, GPI Linked Anchor Protein, GPI-linked Anchor Protein, PI Linked Cell Surface Accessory Protein, RET 1L, RET Ligand 1, RET1L, RETL 1, RETL1, TGF beta Related Neurotrophic Factor Receptor 1, TGF-beta Related Neurotrophic Factor Receptor 1, TRNR 1, TRNR1)
GDNF family receptor alpha-1 (GFRa1), also known as the GDNF receptor or GFRA1, is a protein that in humans is encoded by the GFRA1 gene. It is mapped to chromosome 10q25.3. The protein encoded by this gene is a member of the GDNF receptor family. GFRA1 is released by neuronal cells, Schwann cells, and injured sciatic nerve. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is also a candidate gene for Hirschsprung disease. Soluble GFRA1 mediates robust recruitment of RET to lipid rafts via a mechanism requiring the RET tyrosine kinase.
Applications
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.2ng/lane under reducing conditions. Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human GFRA1 expressed in E. coli. (Position: D25-Q227). Species sequence homology: mouse and rat (97%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human GFRA1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
References
1. Gorodinsky A, Zimonjic DB, Popescu NC, Milbrandt J (March 1998). "Assignment of the GDNF family receptor alpha-1 (GFRA1) to human chromosome band 10q26 by in situ hybridization". Cytogenet Cell Genet 78(3-4): 289–90. 2. Angrist M, Jing S, Bolk S, Bentley K, Nallasamy S, Halushka M, Fox GM, Chakravarti A (June 1998). "Human GFRA1: cloning, mapping, genomic structure, and evaluation as a candidate gene for Hirschsprung disease susceptibility". Genomics 48(3): 354–62. 3. "Entrez Gene: GFRA1 GDNF family receptor alpha 1"USBio References
No references available