Technical Data

303371-ML650
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Conjugate
MaxLight™650
Isotype
IgG
Grade
Affinity Purified
Applications
FLISA WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze
Notes
Preservative Free
BSA Free
Rabbit Anti-I-309 (C-C Motif Chemokine 1, Ccl1, CCL1_HUMAN, Chemokine CC Motif Ligand 1, Inflammatory Cytokine I-309, P500, SCYA1, SISe, Small Inducible Cytokine A1, Small-inducible Cytokine A1, T Lymphocyte Secreted Protein I-309, T Lymphocyte-secreted Protein I-309, TCA3) (MaxLight 650)

MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.

CCL1, Chemokine (C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8.
Applications
Suitable for use in FLISA and Western Blot. Other applications not tested.
Recommended Dilution
FLISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
Immunogen
Recombinant protein corresponding to human I-309 expressed in E. coli. (Position: K24-K96). Species sequence homology: mouse (38%)
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human I-309.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Miller, M. D., Wilson, S. D., Dorf, M. E., Seuanez, H. N., O'Brien, S. J., Krangel, M. S.Sequence and chromosomal location of the I-309 gene: relationship to genes encoding a family of inflammatory cytokines.J. Immun. 145: 2737-2744, 1990.
USBio References
No references available
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