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303389 Rabbit Anti-IL3 (Colony Stimulating Factor Multiple, Hematopoietic Growth Factor, IL 3, IL-3, IL3_HUMAN, Interleukin 3 (Colony Stimulating Factor, Multiple), Interleukin 3, Interleukin-3, Mast Cell Growth Factor, MCGF, Multi CSF, Multilineage Colony Stimulating Factor, Multipotential Colony Stimulating Factor, Multipotential Colony-Stimulating Factor, P Cell Stimulating Factor, P-cell-stimulating Factor)

Specifications
References
Clone Type
Polyclonal
Host
Rabbit
Source
Rat
Swiss Prot
P04823
Isotype
IgG
Grade
Affinity Purified
Applications
E WB
Crossreactivity
Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C

Interleukin 3, also known as IL-3, is a protein that in humans is encoded by the IL3 gene. It is mapped to 5q31.1. IL-3 is an interleukin, a type of biological signal (cytokine) that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin-3 receptor. IL-3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or, with the addition of IL-7, into lymphoid progenitor cells. In addition, IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes, monocytes, and dendritic cells), in conjunction with other cytokines, e.g., Erythropoietin (EPO), Granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6. IL-3 is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response.

Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to rat IL3 expressed in E. coli. (Position: S28-C166). Species sequence homology: human (32%) and mouse (55%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes rat IL3.
References
1. Yang YC, Ciarletta AB, Temple PA,Chung MP, Kovacic S, Witek-Giannotti JS, Leary AC, Kriz R, Donahue RE, Wong GG (October 1986). "Human IL-3 (multi-CSF): identification by expression cloning of a novel hematopoietic growth factor related to murine IL-3". Cell 47(1): 3–10. 2. Webb, G. C., Lee, J. S., Campbell, H .D., Young, I. G.The genes for interleukins 3 and 5 map to the same locus on mouse chromosome 11. Cytogenet. Cell Genet. 50: 107-110, 1989.
USBio References
No references available
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