Technical Data

303562
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu Rt
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-PTEN (Bannayan Zonana Syndrome, BZS, DEC, GLM2, ITGA 2, MHAM, MMAC 1, MMAC1, MMAC1 Phosphatase and Tensin Homolog Deleted on Chromosome 10, Multiple Hamartoma (Cowden Syndrome), Mutated in Multiple Advanced Cancers 1, Mutated in Mutiple Advanced Cancers 1, Phosphatase and Tensin Homolog, Phosphatase and Tensin like Protein, Phosphatidylinositol 345 Trisphosphate 3 Phosphatase and Dual Specificity Protein Phosphatase PTEN, Phosphatidylinositol 345 Trisphosphate 3 Phosphatase, Phosphatidylinositol-3,4,5-trisphosphate 3 Phosphatase and Dual-specificity Protein Phosphatase PTEN, Platelet Antigen BR, PTEN 1, Pten, PTEN_HUMAN, PTEN1, Tensin Homolog, TEP 1, TEP1, VLA 2 Receptor Alpha Subunit)

PTEN is also known as BZS, DEC, CWS1, GLM2, MHAM, TEP1, PTEN1. It is mapped to 10q23.3. This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. The PTEN structure reveals a phosphatase domain that is similar to protein phosphatases but also has an enlarged active site important for the accommodation of the phosphoinositide substrate.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human PTEN expressed in E. coli. (Position: E91-V403). Species sequence homology: mouse (99.7%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human PTEN. Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Lee, J.-O., Yang, H., Georgescu, M.-M., Di Cristofano, A., Maehama, T., Shi, Y., Dixon, J. E., Pandolfi, P., Pavletich, N. P. Crystal structure of the PTEN tumor suppressor: implications for its phosphoinositide phosphatase activity and membrane association. Cell 99: 323-334, 1999.
USBio References
No references available
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