Rabbit Anti-RBP4 (Plasma Retinol Binding Protein 4, Plasma Retinol-binding Protein, Plasma Retinol-binding Protein(1-176), PRBP, RBP, RDCCAS, RET4_HUMAN, Retinol Binding Protein 4, Retinol Binding Protein 4 Interstitial, Retinol Binding Protein 4 Plasma)
RBP4 is also known as RDCCAS. This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells.
Applications
Suitable for use in ELISA and Western Blot. Other applications not tested.
Recommended Dilution
ELISA: 0.1-0.5ug/ml Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human RBP4 expressed in E. coli. (Position: E19-201L). Species sequence homology: mouse and rat (86%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human RBP4. Species Crossreactivity: mouse and rat
