Rabbit Anti-Semaphorin 3A (Coll 1, Coll1, HH16, Hsema I, Hsema III, Sema Domain Immunoglobulin Domain (Ig) Short Basic Domain Secreted (Semaphorin) 3A, Sema III, SEMA1, SEMA1-PEN, SEMA3A, SEMAD, SEMAIII, SEMAL, Semaphorin 3A Precursor, Semaphorin III, Semaphorin Like Growth Cone Guidance Homolog of Chick Collapsin, Semaphorin-3A Precursor, Semaphorin3A, SemD)
SEMA3A is also known as COLL1, SEMA1 or SEMAL. This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease.
Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Recombinant protein corresponding to human Semaphorin 3A expressed in E. coli. (Position: K550-V771). Species sequence homology: mouse (93%) and rat (94%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Semaphorin 3A.
