Technical Data

303702
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-Wnt1, NT (BMND16, INT1, OI15, Oncogene Int1, Proto Oncogene Protein Wnt 1, Proto-oncogene Int-1 Homolog, Proto-oncogene Wnt-1, Wingless Type MMTV Integration Site Family Member 1, Wingless-type MMTV Integration Site Family, Member 1 (Oncogene INT1), Wnt 1, WNT1_HUMAN)

Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.

Applications
Suitable for use in Western Blot. Other applications not tested.
Recommended Dilution
Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions. Optimal dilutions to be determined by the researcher.
Storage and Stability
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunogen
Synthetic peptide corresponding to aa45-74, NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ, from human Wnt1 at N-terminus, identical to the related mouse and rat sequences.
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human Wnt1.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
1. Arheden K, Mandahl N, Strombeck B, Isaksson M, Mitelman F (May 1988). "Chromosome localization of the human oncogene INT1 to 12q13 by in situ hybridization". Cytogenet Cell Genet 47(1–2): 86–87.|2. "Entrez Gene: WNT1 wingless-type MMTV integration site family, member 1". 3. Van Ooyen A, Kwee V, Nusse R (Jan 1986). "The nucleotide sequence of the human int-1 mammary oncogene; evolutionary conservation of coding and non-coding sequences". EMBO J 4(11): 2905–9.
USBio References
No references available
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