Rabbit Anti-ALAS1 (5-aminolevulinate Synthase, Nonspecific, Mitochondrial, ALAS-H, 5-aminolevulinic Acid synthase 1, Delta-ALA Synthase 1, Delta-aminolevulinate Synthase 1, ALAS3, ALASH)
aminolevulinate, delta-, synthase 1
This gene encodes the mitochondrial enzyme which is catalyzes the rate-limiting step in heme (iron-protoporphyrin) biosynthesis. The enzyme encoded by this gene is the housekeeping enzyme; a separate gene encodes a form of the enzyme that is specific for erythroid tissue. The level of the mature encoded protein is regulated by heme: high levels of heme down-regulate the mature enzyme in mitochondria while low heme levels up-regulate. A pseudogene of this gene is located on chromosome 12. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Applications
Suitable for use in ELISA, Immunohistochemistry. Other applications not tested.
Recommended Dilution
ELISA: 1:1000-1:2000 Immunohistochemistry: 1:15-1:50 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Fusion protein of human ALAS1
Form
Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide, 40% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ALAS1. Species Crossreactivity: mouse and rat
