Technical Data

316824
Clone Type
Polyclonal
Host
Rabbit
Source
Human
Isotype
IgG
Grade
Affinity Purified
Applications
IF IHC WB
Crossreactivity
Hu Mo
Gene ID
8888
Shipping Temp
Blue Ice
Storage Temp
-20°C
Rabbit Anti-GANP (Germinal-center Associated Nuclear Protein, 80kD MCM3-Associated Protein, MCM3 acetylating Protein, MCM3 Acetyltransferase, MCM3AP, GANP, KIAA0572, MAP80)
80kD MCM3-associated protein, GANP protein, KIAA0572, MAP80, MCM3A

Isoform GANP:Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells By similarity. Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery. Isoform MCM3AP:Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.

Applications
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Other applications not tested.
Recommended Dilution
Western Blot: 1:500-1:3000 Immunohistochemistry: 1:50-1:100 Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Synthetic peptide corresponding to human GANP.
Form
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 0.02% sodium azide, 50% glycerol.
Purity
Purified by immunoaffinity chromatography.
Specificity
Human Mouse

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

References
Nobuo Sakaguchi, J. Immunol., Apr 2005; 174: 4485 - 4494. Kazuhiko Kuwahara, Blood, Apr 2000; 95: 2321 - 2328. Y Kono, K Maeda, Genes Cells, Aug 2002; 7: 821 - 834. Kazuhiko Kuwahara, PNAS, Aug 2001; 98: 10279.
USBio References
No references available
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