Rabbit Anti-Hyaluronidase PH-20 (SPAM1) (Biotin)
Hyal-PH20, Hyaluronoglucosaminidase PH-20, Sperm adhesion molecule 1, Sperm surface protein PH-20, HYAL3, PH20, SPAM1
Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Applications
Suitable for use in ELISA. Other applications not tested.
Recommended Dilutions
Optimal dilutions to be determined by the researcher.
Storage and Stability
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Immunogen
Recombinant protein corresponding to aa36-490 from human Hyaluronidase PH-20.
Form
Supplied as a liquid in PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol. Labeled with Biotin.
Purity
Purified by Protein G affinity chromatography.
Specificity
Recognizes human Hyaluronidase PH-20.